The systematic analysis of protein-lipid interactions comes of age
2015
Authors: Saliba AE, Vonkova I, Gavin AC
CellNetworks People: Gavin Anne-Claude
Journal: Nat Rev Mol Cell Biol. 2015 Dec;16(12):753-61. doi: 10.1038/nrm4080

Lipids tailor membrane identities and function as molecular hubs in all cellular processes. However, the ways in which lipids modulate protein function and structure are poorly understood and still require systematic investigation. In this Innovation article, we summarize pioneering technologies, including lipid-overlay assays, lipid pull-down assays, affinity-purification lipidomics and the liposome microarray-based assay (LiMA), that will enable protein-lipid interactions to be deciphered on a systems level. We discuss how these technologies can be applied to the charting of system-wide networks and to the development of new pharmaceutical strategies.