PhD position: “Nuclear pore complex biogenesis” Centre for Molecular Biology (ZMBH) Heidelberg University
Category:
Phd

Description:
The nuclear pore complex (NPC) is a large cylindrical structure with multiple copies of over 30 different proteins named nucleoporins (NUPs). The NPC is embedded in the nuclear envelope (NE) at fusion sites of the inner and outer nuclear membrane where it facilitates nuclear-cytoplasmic transport of RNA and proteins. NPCs assemble in the intact nuclear envelope (interphase human cells or yeast cells with a closed mitosis) by an inside-out mechanism. NUPs become deposited from within the nucleus to the inner nuclear membrane, deform this membrane and eventually the inner and outer nuclear membranes fuse. We recently have identified with Brr6 and Brl1 two conserved integral membrane proteins that may play a role in nuclear membrane fusion during NPC biogenesis.

In this project we will study NPC biogenesis using budding yeast and human cells as model systems. The PhD student will use biochemical approaches (Brr6 and Brl1 reconstitution into liposomes), super resolution microscopy (SIM and STED), electron microscopy, CRISPR/Cas9 technology and live cell imaging to study NPC assembly.

Highly motivated PhD students with a background in biochemistry, cell biology or molecular biology should apply. Successful candidates will be part of an international team of PhD students and postdocs that works at the forefront of scientific research. The PhD student will be a member of the Hartmut Hoffmann-Berling International Graduate School of Molecular and Cellular Biology (http://www.hbigs.uni-heidelberg.de). The PhD position is funded for 3 years.

 

Please send applications to E. Schiebel (schiebel.elmar [ aT ] zmbh.uni-heidelberg.de).

 

Relevant recent publications:

1 Zhang, W. et al. Brr6 1 and Brl1 locate to nuclear pore complex assembly sites to promote their biogenesis. J Cell Biol in press (2018).

2 Ruthnick, D. et al. Characterization of spindle pole body duplication reveals a regulatory role for nuclear pore complexes. J Cell Biol 216, 2425-2442, doi:10.1083/jcb.201612129 (2017).

3 Rüthnick, D. & Schiebel, E. Duplication of the yeast spindle pole body once per cell cycle. Mol Cell Biol 36, 1324-1331, doi:10.1128/MCB.00048-16 (2016).

4 Seybold, C. et al. Kar1 binding to Sfi1 C-terminal regions anchors the SPB bridge to the nuclear envelope. J Cell Biol 209, 843-861, doi:10.1083/jcb.201412050 (2015).