Carboxylated Photoswitchable Diarylethenes for Biolabeling and Super-Resolution RESOLFT Microscopy
2016
Authors: Roubinet B, Bossi ML, Alt P, Leutenegger M, Shojaei H, Schnorrenberg S, Nizamov S, Irie M, Belov VN, Hell SW
CellNetworks People: Hell Stefan
Journal: Angew Chem Int Ed Engl. 2016 Dec 5;55(49):15429-15433. doi: 10.1002/anie.201607940

Reversibly photoswitchable 1,2-bis(2-ethyl-6-phenyl-1-benzothiophene-1,1-dioxide-3-yl)perfluorocyclopentenes (EBT) having fluorescent "closed" forms were decorated with four or eight carboxylic groups and attached to antibodies. Low aggregation, efficient photoswitching in aqueous buffers, specific staining of cellular structures, and good photophysical properties were demonstrated. Alternating light pulses of UV and blue light induce numerous reversible photochemical transformations between two stables states with distinct structures. Using relatively low light intensities, EBTs were applied in biology-related super-resolution microscopy based on the reversible saturable (switchable) optical linear fluorescence transitions (RESOLFT) and demonstrated optical resolution of 75 nm.