Fluorogenic Probes for Multicolor Imaging in Living Cells
Authors: Lukinavičius G, Reymond L, Umezawa K, Sallin O, D'Este E, Göttfert F, Ta H, Hell SW, Urano Y, Johnsson K
CellNetworks People: Hell Stefan
Journal: J Am Chem Soc. 2016 Aug 3;138(30):9365-8. doi: 10.1021/jacs.6b04782

Here we present a far-red, silicon-rhodamine-based fluorophore (SiR700) for live-cell multicolor imaging. SiR700 has excitation and emission maxima at 690 and 715 nm, respectively. SiR700-based probes for F-actin, microtubules, lysosomes, and SNAP-tag are fluorogenic, cell-permeable, and compatible with superresolution microscopy. In conjunction with probes based on the previously introduced carboxy-SiR650, SiR700-based probes permit multicolor live-cell superresolution microscopy in the far-red, thus significantly expanding our capacity for imaging living cells.