Dynamic imaging of colloidal-crystal nanostructures at 200 frames per second
Authors: Lauterbach MA, Ullal CK, Westphal V, Hell SW
CellNetworks People: Hell Stefan
Journal: Langmuir. 2010 Sep 21;26(18):14400-4. doi: 10.1021/la102474p

The dynamic noninvasive imaging of colloidal nanostructures has been precluded by the diffraction-limited resolution of (confocal) light microscopy. Using Fast Stimulated Emission Depletion (STED) microscopy, we demonstrate the ability to resolve the formation of a colloidal crystal (monolayer) from particles of 200 nm size, where the voids in the crystal are as small as 30 nm. With a temporal resolution of 5 ms, we exemplify the technique by visualizing the annealing of potential point defects during the formation of the colloidal crystal.