Activation of membrane-permeant caged PtdIns(3)P induces endosomal fusion in cells
|Authors:||Subramanian D, Laketa V, Müller R, Tischer C, Zarbakhsh S, Pepperkok R, Schultz C|
|CellNetworks People:||Pepperkok Rainer|
|Journal:||Nat Chem Biol. 2010 May;6(5):324-6|
Phosphatidylinositol 3-phosphate (PtdIns(3)P) is a phospholipid residing on early endosomes, where it is proposed to be involved in endosomal fusion. We synthesized membrane-permeant derivatives of PtdIns(3)P, including a caged version that is to our knowledge the first photoactivatable phosphoinositide derivative developed so far. In living cells, photoactivation of caged PtdIns(3)P induced rapid endosomal fusion in an EEA1-dependent fashion, thus providing in vivo evidence that PtdIns(3)P is a sufficient signal for driving this process.