Micropilot: automation of fluorescence microscopy-based imaging for systems biology
|Authors:||Conrad C, Wünsche A, Tan TH, Bulkescher J, Sieckmann F, Verissimo F, Edelstein A, Walter T, Liebel U, Pepperkok R, Ellenberg J.|
|CellNetworks People:||Ellenberg Jan, Pepperkok Rainer|
|Journal:||Nat Methods. 2011 Mar;8(3):246-9.|
Quantitative microscopy relies on imaging of large cell numbers but is often hampered by time-consuming manual selection of specific cells. The 'Micropilot' software automatically detects cells of interest and launches complex imaging experiments including three-dimensional multicolor time-lapse or fluorescence recovery after photobleaching in live cells. In three independent experimental setups this allowed us to statistically analyze biological processes in detail and is thus a powerful tool for systems biology.