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Micropilot: automation of fluorescence microscopy-based imaging for systems biology
2011
| Authors: | Conrad C, Wünsche A, Tan TH, Bulkescher J, Sieckmann F, Verissimo F, Edelstein A, Walter T, Liebel U, Pepperkok R, Ellenberg J. |
| CellNetworks People: | Ellenberg Jan, Pepperkok Rainer |
| Journal: | Nat Methods. 2011 Mar;8(3):246-9. |
Quantitative microscopy relies on imaging of large cell numbers but is often hampered by time-consuming manual selection of specific cells. The 'Micropilot' software automatically detects cells of interest and launches complex imaging experiments including three-dimensional multicolor time-lapse or fluorescence recovery after photobleaching in live cells. In three independent experimental setups this allowed us to statistically analyze biological processes in detail and is thus a powerful tool for systems biology.