Rapid control of protein level in the apicomplexan Toxoplasma gondii.
|Authors:||Herm-Götz A, Agop-Nersesian C, Münter S, Grimley JS, Wandless TJ, Frischknecht F, Meissner M.|
|CellNetworks People:||Frischknecht Friedrich, Meissner Markus|
|Journal:||Nat Methods. 2007 Dec;4(12):1003-5.|
Analysis of gene function in apicomplexan parasites is limited by the absence of reverse genetic tools that allow easy and rapid modulation of protein levels. The fusion of a ligand-controlled destabilization domain (ddFKBP) to a protein of interest enables rapid and reversible protein stabilization in T. gondii. This allows an efficient functional analysis of proteins that have a dual role during host cell invasion and/or intracellular growth of the parasite.