Continuous throughput and long-term observation of single-molecule FRET without immobilization
|Authors:||Tyagi S, Vandelinder V, Banterle N, Fuertes G, Milles S, Agez M, Lemke EA|
|CellNetworks People:||Lemke Edward|
|Journal:||Nat Methods. 2014 Jan 19. doi: 10.1038/nmeth.2809. [Epub ahead of print]|
We present an automated microfluidic platform that performs multisecond observation of single molecules with millisecond time resolution while bypassing the need for immobilization procedures. With this system, we confine biomolecules to a thin excitation field by reversibly collapsing microchannels to nanochannels. We demonstrate the power of our method by studying a variety of complex nucleic acid and protein systems, including DNA Holliday junctions, nucleosomes and human transglutaminase 2.