Nanoscopy in a living mouse brain
Authors: Berning S, Willig KI, Steffens H, Dibaj P, Hell SW
CellNetworks People: Hell Stefan
Journal: Science. 2012 Feb 3;335(6068):551. doi: 10.1364/OE.20.005225

We demonstrated superresolution optical microscopy in a living higher animal. Stimulated emission depletion (STED) fluorescence nanoscopy reveals neurons in the cerebral cortex of a mouse with <70-nanometer resolution. Dendritic spines and their subtle changes can be observed at their relevant scales over extended periods of time.