Nanoscopy in a living mouse brain
|Authors:||Berning S, Willig KI, Steffens H, Dibaj P, Hell SW|
|CellNetworks People:||Hell Stefan|
|Journal:||Science. 2012 Feb 3;335(6068):551. doi: 10.1364/OE.20.005225|
We demonstrated superresolution optical microscopy in a living higher animal. Stimulated emission depletion (STED) fluorescence nanoscopy reveals neurons in the cerebral cortex of a mouse with <70-nanometer resolution. Dendritic spines and their subtle changes can be observed at their relevant scales over extended periods of time.