Dual channel RESOLFT nanoscopy by using fluorescent state kinetics
2015
Authors: Testa I, D'Este E, Urban NT, Balzarotti F, Hell SW
CellNetworks People: Hell Stefan
Journal: Nano Lett. 2015 Jan 14;15(1):103-6. doi: 10.1021/nl503058k

We show that RESOLFT fluorescence nanoscopy, a low light level scanning superresolution technique employing reversibly switchable fluorescent proteins (rsFPs), is capable of dual-channel live-cell imaging that is virtually free of chromatic errors and temporal offsets. This is accomplished using rsEGFP and Dronpa, two rsFPs having similar spectra but different kinetics of switching and fluorescence emission. Our approach is demonstrated by imaging protein distributions and dynamics in living neurons and neuronal tissues.