Nanoscopy of filamentous actin in cortical dendrites of a living mouse
Authors: Willig KI, Steffens H, Gregor C, Herholt A, Rossner MJ, Hell SW
CellNetworks People: Hell Stefan
Journal: Biophys J. 2014 Jan 7;106(1):L01-3. doi: 10.1016/j.bpj.2013.11.1119

We demonstrate superresolution fluorescence microscopy (nanoscopy) of protein distributions in a mammalian brain in vivo. Stimulated emission depletion microscopy reveals the morphology of the filamentous actin in dendritic spines down to 40 μm in the molecular layer of the visual cortex of an anesthetized mouse. Consecutive recordings at 43-70 nm resolution reveal dynamical changes in spine morphology.