PerkinElmer UltraVIEW VoX is a fully motorized spinning disc confocal microscope. It is equipped with 6 lasers (405nm, 440nm, 488nm, 514nm, 561nm and 640nm) and 2 cameras (back-illuminated EMCCD Hamamatsu ImagEM X2 and sCMOS Orca Flash4). It is based on a Nikon TiE stand, has hardware-based autofocus (PFS) and environment control box (CO2, temperature) making it suitable for live cell imaging. A FRAP unit is available for photobleaching experiments.
The microscope is part of the Infectious Diseases Imaging Platform, Department of Infectious Diseases, University Hospital Heidelberg, it is located in the INF 325, U21 and placed under enhanced biosafety level (BSL 2).
The microscope is available to other CellNetworks members at the campus requiring BSL 2 imaging infrastructure. Here are some examples of applications suitable for the spinning disc confocal microscopy:
- all experimental setups which involve (fast) imaging of living cells under BSL 2 AND require optical sectioning (due to lower cytotoxicity and higher speed compared to point laser scanning confocal microscopes)
- all experimental setups which involve imaging of fixed cells AND require multichannel (3 channels or more), fine-step, 3D acquisition (stacks of 10 slices or more) (due to high acquisition speed compared to point laser scanning confocal microscopes)
- imaging weakly fluorescent samples in far-red part of the spectrum that require optical sectioning (due to better a quantum efficiency of cameras compared to PMTs in the far-red range).
The microscope is managed through Nikon Imaging Center (NIC) Heidelberg (http://nic.uni-hd.de/) user and booking system. The fee system is described here http://www.nic.uni-hd.de/md/nic/pricelist.pdf.
Dr. Vibor Laketa (e-mail: vibor.laketa [ aT ] med.uni-heidelberg.de, phone 06221-56 4752) from the Department of Infectious Diseases, University Hospital Heidelberg is the contact person for all the aspects concerning this system.
To allow effective reporting to the granting agencies the usage of the system needs to be acknowledged in the enabling publications. Here is an example of the acknowledgement text:
"We would like to acknowledge/thank Infectious Diseases Imaging Platform at the Department of Infectious Diseases, University Hospital Heidelberg for providing "microscopy" support (you can change "microscopy" with something more specific or leave it like that)".
No one is allowed to use the system before receiving the BSL2 training and contacting Dr. Laketa.