Inverted light-sheet microscope for imaging mouse pre-implantation development
2015
Authors: Strnad P, Gunther S, Reichmann J, Krzic U, Balazs B, de Medeiros G, Norlin N, Hiiragi T, Hufnagel L, Ellenberg J
CellNetworks People: Ellenberg Jan
Journal: Nat Methods. 2015 Dec 14. doi: 10.1038/nmeth.3690. [Epub ahead of print]

Despite its importance for understanding human infertility and congenital diseases, early mammalian development has remained inaccessible to in toto imaging. We developed an inverted light-sheet microscope that enabled us to image mouse embryos from zygote to blastocyst, computationally track all cells and reconstruct a complete lineage tree of mouse pre-implantation development. We used this unique data set to show that the first cell fate specification occurs at the 16-cell stage.