A small-molecule FRET probe to monitor phospholipase A2 activity in cells and organisms
2006
Authors: Wichmann O, Wittbrodt J, Schultz C
CellNetworks People: Wittbrodt Joachim
Journal: Angew Chem Int Ed Engl. 2006 Jan 9;45(3):508-12

Watching live: The FRET reporter PENN/SATE (FRET = fluorescence resonance energy transfer) is membrane permeant and enters cells and small organisms by using a prodrug approach. Once inside cells the reporter is cleaved by phospholipases, which results in a 30-fold change in the emission ratio.