Fluorescent Rhodamines and Fluorogenic Carbopyronines for Super-Resolution STED Microscopy in Living Cells
2016
Authors: Butkevich AN, Mitronova GY, Sidenstein SC, Klocke JL, Kamin D, Meineke DN, D'Este E, Kraemer PT, Danzl JG, Belov VN, Hell SW
CellNetworks People: Hell Stefan
Journal: Angew Chem Int Ed Engl. 2016 Mar 1;55(10):3290-4. doi: 10.1002/anie.201511018

A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of λ=500-630 nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1 μm solutions of the dye-ligand conjugates. The synthesis, photophysical parameters, fluorogenic behavior, and structure-property relationships of the new dyes are discussed. Light microscopy with stimulated emission depletion (STED) provided one- and two-color images of living cells with an optical resolution of 40-60 nm.