SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy
2015
Authors: Lukinavičius G, Blaukopf C, Pershagen E, Schena A, Reymond L, Derivery E, Gonzalez-Gaitan M, D'Este E, Hell SW, Gerlich DW, Johnsson K
CellNetworks People: Hell Stefan
Journal: Nat Commun. 2015 Oct 1;6:8497. doi: 10.1038/ncomms9497

Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR-Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy. The combination of these properties makes this probe a powerful tool for live-cell imaging.