Parallelized STED fluorescence nanoscopy
2011
Authors: Bingen P, Reuss M, Engelhardt J, Hell SW
CellNetworks People: Hell Stefan
Journal: Opt Express. 2011 Nov 21;19(24):23716-26. doi: 10.1364/OE.19.023716

We introduce a parallelized STED microscope featuring m = 4 pairs of scanning excitation and STED beams, providing m-fold increased imaging speed of a given sample area, while maintaining basically all of the advantages of single beam scanning. Requiring only a single laser source and fiber input, the setup is inherently aligned both spatially and temporally. Given enough laser power, the design is readily scalable to higher degrees of parallelization m.