Dual-label STED nanoscopy of living cells using photochromism
2011
Authors: Willig KI, Stiel AC, Brakemann T, Jakobs S, Hell SW
CellNetworks People: Hell Stefan
Journal: Nano Lett. 2011 Sep 14;11(9):3970-3. doi: 10.1021/nl202290w

We demonstrate live-cell STED microscopy of two protein species using photochromic green fluorescent proteins as markers. The reversible photoswitching of two markers is implemented so that they can be discerned with a single excitation and STED wavelength and a single detection channel. Dual-label STED microscopy is shown in living mammalian cells.