Nanoscale separation of molecular species based on their rotational mobility
Authors: Testa I, Schönle A, von Middendorff C, Geisler C, Medda R, Wurm CA, Stiel AC, Jakobs S, Bossi M, Eggeling C, Hell SW, Egner A
CellNetworks People: Hell Stefan
Journal: Opt Express. 2008 Dec 8;16(25):21093-104

We combine far-field fluorescence nanoscopy through serialized recording of switchable emitters with polarization-sensitive fluorescence detection. In addition to imaging with nanoscale spatial resolution, this technique allows determination of the fluorescence anisotropy of each detected dipole emitter and thus an estimate of its rotational mobility. Sub-populations of fluorescent markers can thus be separated based on their interaction with the sample. We applied this new functional nanoscopy to imaging of living mammalian cells.