Fluorescence nanoscopy by ground-state depletion and single-molecule return
|Authors:||Fölling J, Bossi M, Bock H, Medda R, Wurm CA, Hein B, Jakobs S, Eggeling C, Hell SW|
|CellNetworks People:||Hell Stefan|
|Journal:||Nat Methods. 2008 Nov;5(11):943-5. doi: 10.1038/nmeth.1257|
We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine- and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach.