SuReSim: simulating localization microscopy experiments from ground truth models
|Authors:||Venkataramani V, Herrmannsdörfer F, Heilemann M, Kuner T.|
|CellNetworks People:||Heilemann Mike, Kuner Thomas|
|Journal:||Nat Methods. 2016 Apr;13(4):319-21. doi: 10.1038/nmeth.3775. Epub 2016 Feb 29.|
Super-resolution fluorescence microscopy has become a widely used tool in many areas of research. However, designing and validating super-resolution experiments to address a research question in a technically feasible and scientifically rigorous manner remains a fundamental challenge. We developed SuReSim, a software tool that simulates localization data of arbitrary three-dimensional structures represented by ground truth models, allowing users to systematically explore how changing experimental parameters can affect potential imaging outcomes.