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May 17 2018 / 14:15 PM

Category:
Life Science

Lecturer:
Prof. Dr. Pierre Gönczy, Swiss Institute for Experimental Cancer Research, School of Life Sciences, École polytechnique fédérale de Lausanne (EPFL), Lausanne, Switzerland

Place:
Institute for Theoretical Physics, Seminar room, Philosophenweg 19, 69120 Heidelberg

Host:
Ulrich Schwarz

Description:
The centriole is a remarkable microtubule-based organelle that is essential for the formation of cilia, flagella and centrosomes. The centriole is organized around a nine-fold symmetrical cartwheel typically ~100 nm in height, which is critical for the onset of organelle biogenesis. The cartwheel comprises a stack of ring-containing entities that each accommodates nine homodimers of SAS-6 proteins. In contrast to the knowledge about the self-assembly properties of SAS-6 proteins, the mechanisms enabling ring stacking are poorly understood. Furthermore, the assembly dynamics of SAS-6 ring-containing entities remains elusive. After introducing the subject matter, I will report notably on our development of a cell-free assay to address this important open question using the Chlamydomonas reinhardtii SAS-6 protein CrSAS-6. Using high-speed atomic force microscopy (AFM)-based, we monitored the assembly dynamics of CrSAS-6 homodimers, and thus determined possible routes and kinetic rates for ring formation.

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